Table 1.
Sequences of primers used to amplify fragments from Hypoderaeum conoideum
| Primer codes | Sequences(5′ to 3′) | Target gene |
|---|---|---|
| XCCOX3F2 | AGYACDGTDGGDTTRCATTT | cox31 |
| XCCOX3R1 | CANAYATAATCMACARAATGNCA | cox31 |
| XcND4F | GADTCBCCDTATTCDGARCG | nad41 |
| XcND4R | GCHARCCADCGCTTVCCNTC | nad41 |
| TXCCOX1F | GGHTGAACHRTWTAYCCHCC | cox11 |
| TXCCOX1R | TGRTGRGCYCAWACDAYAMAHCC | cox11 |
| Insect12SF | AAWAAYGAGAGYGACGGGCG | rrnS1 |
| Insect12SR | TARACTAGGATTAGATACCC | rrnS1 |
| XcND5F | ATGCGNGCYCCNACNCCNGTDAG | nad51 |
| XcND5R1 | TGCTTVSWAAAAAANACHCC | nad51 |
| XCF2 | TATTAGGAGGTTTGGTGG | cox3-nad42 |
| XCR3 | ATCATAACTACCACATACCCC | cox3-nad42 |
| XCF4 | TAGGTATTGCTTGTTAGCTG | nad4-cox12 |
| XCR2 | TTTAATCGAACCAAGGACAC | nad4-cox12 |
| XCF3 | CATTAGTCACATTTGTATGAC | cox1- rrnS2 |
| XCR10 | GGACTATCTTTTATGATACACG | cox1- rrnS2 |
| XCF1 | GTTATTGGGTTTAGGACTCGG | rrnS - nad52 |
| XCR8 | ACTAACACCGTATTCAACTC | rrnS - nad52 |
| XCF9 | TTTCTCTTTGTGGTTTGCCG | nad5-cox32 |
| XCR1 | TATTAGGTTGTGGTACCCC | nad5-cox32 |
Primer pairs (top to bottom) used to amplify fragments; 1short regions amplified by PCR from cox1 (494 bp), cox3 (140 bp), nad4 (440 bp), nad5 (529 bp) and rrnS (383 bp). 2large fragments that were amplified by long-range PCR from cox3-nad4 (2048 bp), nad4-cox1 (4664 bp), cox1-rrnS (2352 bp), rrnS-nad5 (2272 bp) and nad5-cox3 (1752 bp).