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. 2015 Feb 12;15:47. doi: 10.1186/s12870-015-0445-1

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© Goetze et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Verification of PsOep23 orientation in a lipid bilayer. The I/V relationship revealed a slightly rectifying current of all open states. This rectification helps to identify the orientation of the channel after fusion with the bilayer, which was randomly distributed. For clarity the large conductance was always allocated to positive holding potentials. The data points represent the average of 16 independent experiments. Electrolyte solution 250 mM KCl, 50 mM Mops/Tris pH 7.0 (symmetrical cis/trans).