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. 2015 Feb 12;15:47. doi: 10.1186/s12870-015-0445-1

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© Goetze et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Channel behavior of PsOep23 in presence of spermine. Current trace of a single active Oep23 channel at a holding potential of (A) +100 and (B) -100 mV in control conditions and after addition of 5 and 10 mM of spermine, respectively. The presence of spermine induced a concentration dependent reduction of the open channel conductance (o - > o’) and the open probability, apparent by a more frequent and prolonged likelihood to remain in a closed or blocked state. This block is completely reversible by washing out spermine (data not shown).