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. Author manuscript; available in PMC: 2015 Feb 17.
Published in final edited form as: Mol Cell Neurosci. 2014 Jun 2;61:123–132. doi: 10.1016/j.mcn.2014.05.007

Fig. 5.

Fig. 5

Striatal DAT protein levels in HAD-Tg mice that lack endogenous DAT. (A) Representative western blot showing DAT protein levels in wildtype mice (WT, n = 3), heterozygous (DAT-HET, n = 3) mice and HAD-Tg mice that lack endogenous DAT (DAT-KO/HAD-Tg, n = 3). A DAT-KO sample is shown as a control for immunoreactivity (n = 1). (B) Densitometric analysis of DAT protein levels. Mean +/− SEM (p ≤ 0.01, **; p ≤ 0.001, ***) (F2,6 = 30.40, p = 0.0007, one-way ANOVA). (C) Uptake of 3H[DA] into striatal synaptosomes of WT and DAT-KO/HAD-Tg mice. Mean values for Vmax shown (pmol per min per mg protein). The average Vmax of DAT-KO controls was deducted from all WT and DAT-KO/HAD-Tg values (n = 3 for both genotypes). Mean +/− SEM, p = 0.038.