Type Vb plaque from the ICA of a 72 year-old, asymptomatic male (patient C). B-mode image (a) shows a complicated lumen with a large area of shadowing (a, white arrows) from plaque in the distal wall. ARFI imaging (b) shows lower displacement in the area of the calcium deposit (asterisk) and higher displacements in the adjacent tissue connecting to the proximal wall (dagger). ARFI magnification image is shown in (c) with a white outline indicating the plaque that is above the shadowed region. Histological staining with H&E (d) and LMT for collagen (e) shows a large calcification (asterisk) indicated by the black stain. The dotted black lines indicate the extent of the ARFI FOV. Magnification insets shows that the tissue connecting the calcification to the proximal wall is comprised of loosely-packed (d.1) and disorganized (e.1) collagen fibers. ICA, internal carotid artery; CCA, common carotid artery; PW, proximal wall (with respect to transducer); DW, distal wall (with respect to transducer).