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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Glia. 2014 Sep 24;63(4):531–548. doi: 10.1002/glia.22754

Fig. 3. Fluorescent in situ hybridization confirms the localization of oligodendrocyte-specific transcripts in Cx3cr1+/GFP microglia.

Fig. 3

(A) RNA FISH reveals oligodendrocyte-specific (Mbp, Mobp, Mag) mRNA punctae (red) in 34-91% (B) of GFP+ microglia. Representative images are shown with insets of GFP-positive microglia as well as GFP-negative cells containing mRNA molecules. Scale bar, 50μm. (C) A representative high resolution confocal z-stack projection demonstrates Mag mRNA puncta in GFP-positive microglia. Arrow points to the co-localization of mRNA and GFP fluorescence (yellow color). x/y, x/z and y/z projections are shown to confirm the intracellular localization of mRNA within a microglial cell body. Scale bar, 5μm. (D) Immunofluorescence analysis of paraformaldehyde-fixed tissue cryosections using Mobp antibodies (red) and endogenous GFP (green) shows that the Mobp mRNA present in Cx3cr1+/GFP microglia is not translated (arrows). The nuclei were counterstained with DAPI (blue). Representative images are shown. Scale bar, 50μm.