Table I.

Nineteen genes evaluated

Gene Tested	Gene Name	Primer 1	Primer 2
Collagen type I	COL1A1	5′-CCT GCG TGT ACC CCA CTC A-3′	5′-ACC AGA CAT GCC TCT TGT CCT T-3′
Collagen type II	COL2A1	5′-TTG CCT ATC TGG ACG AAG CA-3′	5′-TCA TTG GAG CCC TGG ATG A-3′
Collagen type X	COLlOAl	5′-CCA AAG CTT ACC CAG CAA TAG G-3′	5′-TGC TGT TGC CTG TTA TAC AAA ATT T-3′
Metalloproteinase 1	MMP1	5′-TTT GAT GGA CCT GGA GGA AAT C-3′	5′-TGA GCA TCC CCT CCA ATA CC-3′
Metalloproteinase 3	MMP3	5′-CCT GGT ACC CAC GGA ACC T-3′	5′-GGA CAA AGC AGG ATC ACA GTT G-3′
Metalloproteinase 9	MMP9	5′-GAT CCA AAA CTA CTC GGA AGA CTT G-3′	5′-GAA GGC GCG GGC AAA-3′
Metalloproteinase 13	MMP13	5′-ATT AAG GAG CAT GGC GAC TTC T-3′	5′-CCC AGG AGG AAA AGC ATG AG-3′
TIMP1	TIMP1	5′-CAC CCA CAG ACG GCC TTC T-3′	5′-TGG TGT CCC CAC GAA CTT G-3′
TIMP2	TIMP2	5′-CAC CCA GAA GAA GAG CCT GAA-3′	5′-GGC AGC GCG TGA TCT TG-3′
TIMP3	TIMP3	5′-CCA GGA CGC CTT CTG CAA-3′	5′-AGC TTC TTC CCC ACC ACC TT-3′
Interleukin 1β	IL1B	5′-CGA ATC TCC GAC CAC CAC TAC-3′	5′-TCC ATG GCC ACA ACA ACT GA-3′
Interleukin 6	IL6	5′-TGT AGC CGC CCC ACA CA-3′	5′-GGA TGT ACC GAA TTT GTT TGT CAA-3′
Interleukin 17	IL17	5′-TGA TGG GAA CGT GGA CTA CCA-3′	5′-TGC GCA GGA CCA GGA TCT-3′
Versican	VCAN	5′-GCC TAC TTT GCC ACC CAG TTA C-3′	5′-TTT TGT CCA CTT CAA TCT TAG ACC AT-3′
Cyclooxygenase-2	PTGS2	5′-CAG CAC TTC ACG CAT CAG TTT T-3′	5′-CCA GCC CGT TGG TGA AAG-3′
iNOS	NOS2	5′-TGG ATG CAA CCC CAT TGT C-3′	5′-CGC TGC CCC AGT TTT TGA T-3′
TNFα	TNF	5′-CTC GAA CCC CGA GTG ACA A-3′	5′-AGC TGC CCC TCA GCT TGA-3′
ADAMTS5	ADAMTS5	5′-AAT AAC CCT GCT CCC AGA AAC A-3′	5′-GCG GTA GAT GGC CCT CTT C-3′
Connexin 43	GJA1	5′-AAC AAT TCT TCT TGC CGC AAT TAC-3′	5′-CAT TCG ATT TTG TTC TGC ACT GTA-3′

iNOS, inducible nitric oxide synthase; TIMP, tissue inhibitors of metalloproteinase; ADAMTS5, aggrecanase-2; TNFα, tumor necrosis factor alpha.