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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Cell Transplant. 2013 Dec 30;24(2):235–245. doi: 10.3727/096368913X676204

Figure 4.

Figure 4

Self-Assembled Neocartilage ECM Analysis. (A) Neocartilage from each treatment group was analyzed histologically for total collagen, GAGs, type I collagen, and type II collagen. Notably, GAG content varied minimally. With increasing aggregation duration, type I collagen decreased. Scale bar = 100 μm. (B) Neocartilage formed following 14 and 21 days aggregation had the greatest total collagen content, and 14 days significantly increased type II collagen synthesis per cell compared with 0 days. (C) GAG content did not vary appreciably up to 14 days but decreased after 21 days of aggregation. Data are reported as mean ± standard deviation. All groups not connected by a common letter are significantly different (p<0.05).