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. Author manuscript; available in PMC: 2016 Feb 9.
Published in final edited form as: Alcohol Clin Exp Res. 2015 Feb 9;39(2):221–231. doi: 10.1111/acer.12615

Table 2.

Stereology sampling scheme used with the optical disector method to quantify neurons of the cerebral cortex and hippocampal formation.

Cerebral cortex CA3 pyramidal
cells
CA1 pyramidal
cells
Dentate granule
cells
Disector height 12 µm 12 µm 12 µm 12 µm
Counting frame area 25 µm x 25 µm 18 µm x 18 µm 16 µm x 16 µm 10 µm x 10 µm
Raster pattern sampling step 1/238 1/18 1/15 1/40
x,y step 148,750 µm2 5832 µm2 3840 µm2 4000 µm2
Section sampling fraction for Vref 1/4 1/4 1/4 1/4
Section sampling fraction for Nv 1/24 1/12 1/12 1/12