Table 4.

Mean number of alterations observed in A: biofilm and B: cell-free supernatant samples.

A: biofilm samples	Mean number of alterations
Types of alterations	NC1	NC2	NC3	S.mutans BHIM	S.mutans BHIM+3%	C. albicans	P. eruginosa	S. aureus	C.albicans+Str.mutans
Cavities	40.5±8.68	48.83±12.25	89.5±27.61	135.833±29.38	428.5±75.95	201.5±52.75	160.66±42.44	172±44.14	298±127
Pits	0	0	0	0	42.5±15.28	0	0	0	15.5±9
Deep Recesses	0	0	0	31±14.31	6±3.05	64.5±24.5	50.16±19.21	27.66±15.31	288.83±52.55
Vast Depressions	0	0	0	3.16±1.95	13.5±5.5	7.16±3.48	0	6±3.52	0
Spherules	0	0	0	15.33±13.31	0	10±7.23	0	6.33±3.93	0
Acclivities	0	0	0	0	0	0	142.16±61.37	0	0

B: cell-free supernatant	Mean number of alterations
Types of alterations	S.mutans BHIM	S.mutans BHIM+3%	C.albicans	P.aeruginosa	S.aureus	C. albicans+Str. mutans
Cavities	78.83±13.33	179.16±44.95	206.83±73.19	178.16±	139.33±81.12	200.83±42.57
Pits	0	8±5.01	0	0	0	6.66±5.85
Deep Recesses	37.66±20.56	180.16±78.38	50±22.943	98.66±81.98	138.33±157.29	327.33±93.64
Vast Depressions	10±3.46	4.33±2.49	7±2.82	0	10.16±6.7	4,83±3.31
Spherules	23.33±	0	12±8.64	0	15.83±4.49	0
Acclivities	0	0	0	177.33±49.36	0	0

± standard deviation of mean. BHIM – brain-heart infusion media; +3%S – BHIM supplemented with 3% sucrose; NC1,2,3 – negative controls