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. 2015 Feb 7;15:18. doi: 10.1186/s12906-015-0525-7

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© Low et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Chromatograms of standardised A. paniculata extracts. A) The chromatograms of twelve batches (#1 at the bottom, #12 at the top) standardised to contain 30% (w/w) andrographolide were recorded at 227 nm. B) Composite chromatograms illustrating the variation between the HPLC profiles of the 12 batches. The black chromatogram was generated using the highest peaks from each of the 12 extracts. The red chromatogram was generated using the smallest peaks. Vertical blue lines indicate the retention time of the peaks that were used for the quantitative analysis of batch-to-batch variation (see Table 1). Numbered peaks were identified as chlorogenic acid (#1), isoquercetin (#2), andrographolide (#12), andropanoside (#13), apigenin (#15), wogonoside (#16), neoandrogrpapholide (#19), deoxyandrographolide (#23), and 14-deoxy-11,12-didehydroandrographolide (#24).