Table 2.
Primer sequences used in strain generation, verification, sequencing
| Name | Used for | 5′ - 3′ sequence |
|---|---|---|
| kanMX4::LEU2 - fwd | Replace genomic kanMX4 with LEU2 (from pACT2) | GCTGCAGGTCGACGGATCCCCGGGTTAAT TAAGGCGCGCCAGATCAACTGTGGGAATACTCAGGT |
| kanMX4::LEU2 - rev | Replace genomic kanMX4 with LEU2 (from pACT2) | TTTATTGTCAGTACTGATTAGAAAAACTCATCGAGCATC AAATGATCATGATTTTCTGTTACACC |
| LEU2 – fwd | Downtag amplification and sequencing in double mutants | AGGTGTAACAGAAAATCATGAT |
| pFA6 – fwd | Downtag sequencing primer (kanMX4) | CTCGACATCATCTGCCCAGA |
| pFA6 – rev | Uptag sequencing primer (kanMX4) | GTCTGCAGCGAGGAGCCGTA |
| Uptag | Barcode forward primer | GATGTCCACGAGGTCTCT |
| Downtag | Barcode reverse primer | CGGTGTCGGTCTCGTAG |
| Tal1::HPH1-fwd | Replace genomic TAL1 with hphMX4 (from pAG32) | TAGTAAAATACTTCTCGAACTCGTCACATATACGTGTACATAAGCTTGCCTTGTCCCCGCCG |
| Tal1::HPH1-rev | Replace genomic TAL1 with hphMX4 (from pAG32) | GCATAAGGACATGGCCTAAATTAATATTTCCGAGATACTTCCTCGACACTGGATGGCGGCGT |
| NQM1::NTC-fwd | Replace genomic NQM1 with natMX4 (from pAG25) | CCATCTAGAATGGGGTGGACAACATATAAAAGAAGAG |
| NQM1::NTC-rev | Replace genomic NQM1 with natMX4 (from pAG25) | TACGTCAGAATTTTAATGAATATATAAGTCTGTACAC |