Table 3.

The two-way ANOVA analysis for the measured parameters.

Parameter	Source of variation	df	Sum of squares	Mean squares	Variance explained (%)	F	P
Concentration of ions
N-NO3 −	Treatment	2	1908.79	954.40	83	989.3	P < 0.001
	Time	3	158.20	52.73	7	54.6	P < 0.001
	Treatment × time	6	204.12	34.02	9	35.3	P < 0.001
N-NH4 +	Treatment	2	819.2	409.6	64	2275.5	P < 0.001
	Time	3	282.2	94.1	22	522.6	P < 0.001
	Treatment × time	6	13.9	28.9	13	161.1	P < 0.001
Number of nitrifying bacteria	Treatment	2	15.65	7.83	74	124.7	P < 0.001
	Time	3	2.23	0.74	10	11.8	P < 0.001
	Treatment × time	6	1.63	0.27	8	4.3	P = 0.004
AOA-DGGE analysis
H	Treatment	2	0.21	0.11	64	61.2	P < 0.001
	Time	3	0.02	0.01	8	4.8	P = 0.009
	Treatment × time	6	0.05	0.001	16	5.1	P = 0.002
S	Treatment	2	24.50	12.25	72	73.5	P < 0.001
	Time	3	0.66	0.22	2	1.3	P = 0.286
	Treatment × time	6	4.83	0.81	14	4.8	P = 0.002
E H	Treatment	2	0.002	0.0009	24	7.1	P = 0.004
	Time	3	0.001	0.0004	15	3.0	P = 0.052
	Treatment × time	6	0.002	0.0003	20	2.0	P = 0.112
AOB-DGGE analysis
H	Treatment	2	0.52	0.26	34	214.6	P < 0.001
	Time	3	0.46	0.15	30	126.3	P < 0.001
	Treatment × time	6	0.53	0.09	35	72.9	P < 0.001
S	Treatment	2	7.72	3.86	28	139.0	P < 0.001
	Time	3	9.42	3.14	34	113.0	P < 0.001
	Treatment × time	6	9.83	1.63	36	59.0	P < 0.001
E H	Treatment	2	0.0012	0.0005	10	2.5	P = 0.102
	Time	3	0.0009	0.0003	8	1.4	P = 0.267
	Treatment × time	6	0.0039	0.0007	36	3.1	P = 0.023

AOA: ammonia-oxidizing archaea; AOB: ammonia-oxidizing bacteria; H: Shannon-Wiener index; S: richness; E _H: evenness. The effects in bold are significant at P < 0.05.