Table 4. Effect of HZ08 on reversing KB-WT cell and ABCB1-medicated MDR KB-VCR cells.
| Compound | KB-WT Cell | KB-VCR Cell | |||
|---|---|---|---|---|---|
| IC50 (μM) | FR | IC50 (μM) | FR | ||
| VCR | 0.0015±0.0002 | 1.00 | 0.6187±0.1576 | 1.00 | |
| +10 μM VER | 0.0008±0.0000** | 1.83 | 0.0075±0.0015** | 82.74 | |
| +10 μM HZ08 | 0.0004±0.0002** | 3.67 | 0.0025±0.0010** | 246.35 | |
| +5 μM HZ08 | 0.0009±0.0004 | 1.65 | 0.0036±0.0004** | 173.46 | |
| +2.5 μM HZ08 | 0.0024±0.0021 | 0.64 | 0.0147±0.0097** | 42.09 | |
| PTX | 0.0037±0.0009 | 1.00 | 0.1935±0.0177 | 1.00 | |
| +10 μM VER | 0.0018±0.0004* | 2.03 | 0.0094±0.0010*** | 20.60 | |
| +10 μM HZ08 | 0.0014±0.0004* | 2.68 | 0.0015±0.0000*** | 131.04 | |
| +5 μM HZ08 | 0.0019±0.0005* | 1.97 | 0.0016±0.0007*** | 120.06 | |
| +2.5 μM HZ08 | 0.0020±0.0004* | 1.85 | 0.0184±0.0085** | 10.50 | |
| DOX | 0.0473±0.0197 | 1.00 | 0.2447±0.0398 | 1.00 | |
| +10 μM VER | 0.0372±0.0171 | 1.27 | 0.0763±0.0068** | 3.21 | |
| +10 μM HZ08 | 0.0399±0.0166 | 1.19 | 0.0512±0.0103** | 4.78 | |
| +5 μM HZ08 | 0.0573±0.0264 | 0.83 | 0.0637±0.0166** | 3.84 | |
| +2.5 μM HZ08 | 0.0461±0.0251 | 1.03 | 0.0757±0.0278** | 3.23 | |
Data are presented by mean±SD of three independent experiments performed in quadruple. Fold-resistance (FR) was calculated by dividing IC50 value for cells with chemotherapeutic drugs without HZ08 or VER by that obtained in the presence of these two drugs.
* P<0.05
** P<0.01
***P< 0.001 compared to the value obtained without HZ08 or VER.