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. 2015 Feb 10;11:6. doi: 10.1186/s13007-015-0050-1

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© Rousseau et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Bimodal imaging of the embryo of a dry seed of sugar beet with a low spatial resolution of 0,187 mm per isotropic voxel in MRI (A external 3D view and C medial 2D slice) and high spatial resolution of 7,84 μ m per isotropic voxel in X-ray tomography (B external 3D view and D medial 2D slice). The MRI is a spin-echo sequence giving gray-level propotional to the lipid content of the embryo. The red line in panel D is positionned manually on the X-ray at the separation between cotyledon and radicle. Red line in panel C is automatically positioned after registration of both imaging modalities with the ImageJ plugin TrakEM2 of Table 2.