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. 2015 Feb 17;10(2):e0116509. doi: 10.1371/journal.pone.0116509

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© 2015 Bussière et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) Effect of SB203580 on sporozoite trail deposits during parasite gliding. Treatment: sporozoites placed on glass slides covered with gelatin were incubated for 1 h in the presence of SB203580 (25 μM) or DMSO. Pre-treatment: Sporozoites were first treated with SB203580 (25 μM) or DMSO for 1 h; after chasing, the sporozoite suspension was placed on glass slides for 1 h. After fixation, deposited molecules forming trails were revealed by immunofluorescence using a mouse polyclonal antibody against E. tenella sporozoites. Pictures are representative of 3 independent experiments. (B) Effect of SB203580 on sporozoite motility. Sporozoites were treated or pre-treated as described in (A). In this experiment, sporozoite gliding motility was assessed by videorecording and quantified by counting motile sporozoites over a period of 30 s. Data represent the mean of 6 independent experiments ± SEM. Statistical significance was calculated using the Student t test: *P < 0.05.