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. 2015 Feb 17;10(2):e0116509. doi: 10.1371/journal.pone.0116509

Fig 4. SB203580 decreases parasite micronemal protein secretion.

Fig 4

Treatment consisted in incubation of sporozoites for 2 h in complete cell culture medium with SB203580 (25 μM) or DMSO. Pre-treatment consisted in first incubating sporozoites with SB203580 (25 μM) or DMSO for 1 h. Then, after chasing, sporozoites were incubated in complete cell culture medium for 2 h. After incubation, sporozoites were fixed for detection of micronemal proteins onto the surface of the parasite by immunofluorescence (A) or centrifuged for detecting micronemal proteins secreted in the medium and present in sporozoites (pellet) by western blotting (B). (A) Effect of SB203580 on micronemal protein distribution onto the surface E. tenella. This was determined by immunofluorescence. Sporozoites in PBS at 4°C were used as a negative control. (B) Effect of SB203580 on micronemal protein secretion. This was evaluated by western blotting in the supernatants and parasite lysates. Profilin was used as a control of the number of sporozoites. Quantification of secreted micronemal proteins in supernatants was performed using Bio-Profil Bio-1D++ software and is represented by histograms compared to the micronemal protein content in sporozoites. Data are representative of 2 experiments.