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. 2015 Feb 17;10(2):e0117780. doi: 10.1371/journal.pone.0117780

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© 2015 Steinhilber et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — MiRNA expression profiling of the three ALK+ ALCL cell lines SUDHL-1, KiJK and Karpas 299 with (pF-C/EBPβ) and without (pF) C/EBPβ down-regulation. (A) The circle diagram indicates the number of miRNAs regulated by C/EBPβ knockdown in the particular ALK+ ALCL cell line. (B) C/EBPβ-signature of SUDHL-1 cells derived by C/EBPβ-shRNA. Heatmap shows the expression pattern of the 53 miRNAs significantly regulated by C/EBPβ in SUDHL-1 cells, depicting transduced cells with C/EBPβ-shRNA and controls in triplicates. (C) The Venn diagrams illustrate the number of significantly down-regulated (left) and up-regulated (right) miRNAs in the ALK+ ALCL cell lines after transduction with C/EBPβ-shRNA. (D) The table shows from all miR-181 family members the miRNA expression levels (base mean of triplicates) of the three ALK+ ALCL cell lines SUDHL-1, KiJK and Karpas 299 with (pF-C/EBPβ) and without (pF) C/EBPβ knockdown as well as the ALK- ALCL cell line Mac-1 and normal T cells. (E) RT-qPCR analysis of miR-181a and miR-181c expression in ALK+ (SUDHL-1, KiJK and Karpas 299) and ALK- (Mac-1) ALCL cell lines and T cells. Error bars indicate standard deviation of triplicates for cell lines and number of tested samples, respectively. For RT-qPCR quantification values were normalized to miR-106b and data were analysed according to the 2^-ΔΔCp method. Results are depicted as miRNA levels relative to mean value of T cell levels. For statistical analysis of RT-qPCR results a Wilcoxon rank-sum test was used (*p<0.05).