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. 2015 Feb 17;10(2):e0117859. doi: 10.1371/journal.pone.0117859

Fig 2. Mitotic ER rearrangements do not occur until after NEB.

Fig 2

(A) An embryo expressing Rtnl1-GFP and mCh-Tub was observed during cycle 11. Tubulin entered the nuclear space at the 20 second time-point, signaling nuclear envelope breakdown prior to accumulation of ER at the centrosome. At the 40 second time point ER began its rearrangement at the centrosome (arrow). Yellow dotted line denotes fluorescence trace shown in B. Scale bar is 5 μm. (B) Fluorescence intensity traces of Rtnl1 (green line) and mCh-Tub (red line). mCh-Tub displays an intensity peak at 5 μm and 10 μm before NEB (arrowheads). At 20 seconds, mCh-Tub intensity becomes flat indicating NEB, while Rtnl1 intensity begins to form peaks at 5 μm and 10 μm (arrows). Rtnl1 intensity continues to rise and mCh-Tub intensity also rises between 5 μm and 10 μm indicating mitotic spindle formation. Time is in min:sec.