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. 2015 Feb 17;10(2):e0117859. doi: 10.1371/journal.pone.0117859

Fig 8. Cyclin A is sufficient to drive mitotic ER reorganization events.

Fig 8

(A) Schematic of injection strategy and imaging of Drosophila embryo experiment. Pdi-GFP / H2-RFP transgenic embryos were injected with a mixture of APH and CHX, inducing a cycle 11 interphase arrest. Following this arrest, embryos were injected with an affinity-purified recombinant form of cyclin and observed for changes in ER localization. (B) After injection of GST-CycA, ER (green) gathered near the spindle (yellow arrowhead). Pdi-GFP intensity increases much like WT embryos (arrow). Chromosomes (red) eventually condensed and aligned at the metaphase plate (black arrowhead). The spindle region extended into a fusiform structure, but did not progress beyond this point. There was a lack of ER gathering at spindle poles, as well. (C) Following injection of GST-CycB, embryos remained in an interphase-like state without rearrangement of ER (green) or chromosome (red) condensation. Scale bars are 5 μm. Time is in min:sec.