Table 3. The oligonucleotides used for the preparation of the DNA libraries.
Oligonucleotide | Sequence (5'-3') |
---|---|
RE-specific primers | |
AY107 | TCACCGTTTTAGCCGGGA |
AY24 | AGGCGTGAGCCACCGCGC |
AY18 | GAGCCACCGCGCCCGGC |
3-L1HS | GAGATATACCTAATGCTAGATGACAC |
3-end-L1 | GCACATGTACCCTAAAACTTAGAGTA |
Suppression PCR primers and adapters | |
Na21st19 | TGTAGCGTGAAGACGACAGAAAGGGCGTGGTGCGGAGGGCGGT |
st20 | ACCGCCCTCC |
Na15Na21 | AGCAGCGAACTCAGTACAACATGTAGCGTGAAGACGACAGAA |
Na15 | AGCAGCGAACTCAGTACAACA |
st19 | AGGGCGTGGTGCGGAGGGCGGT |