Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 7;22(1):12. doi: 10.1186/s12929-015-0118-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Huang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Effects of PUFAs on cell viability, CRP secretion, and CRP mRNA expression in HepG2 cells. (A) Cell viability was measured by MTT assay after 24 h of PUFA treatment and after a further 24 h of IL-1β/IL-6-stimulation. Cell viability is expressed as a percentage relative to the control value from cells without PUFA treatment and cytokine challenge. (B) Cells were treated with 10 ng/mL of IL-1β/IL-6 for 24 h followed by 100 μM PUFAs for another 24 h. The amount of CRP released into the cell medium was determined by western blot analysis. Pretreatment of HepG2 cells with DHA and EPA resulted in an inhibition of CRP mRNA expression as measured by RT-PCR (C) and real-time quantitative PCR (D). Data are expressed as the mean ± SEM of at least three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001 compared to the IL-1β/IL-6-treated cells.