Table 2.
Summary of the regulated proteins present in the liver of apoE KO mice treated with different PUFAs
| Spot no. (a) | Protein (b) | Ratio PUFA/control (c) | Accession number (b) |
|---|---|---|---|
| Metabolism | |||
| D13 | Fumarylacetoacetase | 3.3 | gi|544273 |
| D14 | Fructose-1,6-bisphosphatase 1 | 2.9 | gi|14547989 |
| D17 | Malate dehydrogenase | 2.6 | gi|126889 |
| D20 | Oxysterols receptor LXR alpha | 2.3 | gi|12644486 |
| D25 | Aldehyde dehydrogenase | 2.1 | gi|1352250 |
| D26 | Isocitrate dehydrogenase | 2.1 | gi|6647554 |
| D31 | SREBP-1 | 0.5 | gi|7144550 |
| D32 | Pyruvate carboxylase | 0.5 | gi|6679237 |
| E8 | ATP synthase beta chain | 3.1 | gi|20455479 |
| E9 | Malate dehydrogenase | 2.8 | gi|126889 |
| E10 | Isocitrate dehydrogenase | 2.6 | gi|6647554 |
| E22 | Alpha enolase | 2.0 | gi|13637776 |
| E23 | Fructose-1,6-bisphosphatase 1 | 2.0 | gi|14547989 |
| E24 | Pyruvate carboxylase | 0.5 | gi|6679237 |
| E25 | SREBP-1 | 0.5 | gi|7144550 |
| A10 | Pyruvate kinase isozyme M2 | 2.6 | gi|2506796 |
| A12 | Malate dehydrogenase | 2.2 | gi|126889 |
| A13 | Glycerol-3-phosphate dehydrogenase | 2.1 | gi|121557 |
| A14 | Ketohexokinase | 2.1 | gi|6016435 |
| L3 | Succinyl-CoA synthetase | 4.8 | gi|52788305 |
| L9 | Fructose-1,6-bisphosphatase 1 | 3.4 | gi|14547989 |
| L10 | Fumarylacetoacetase | 3.2 | gi|544273 |
| L14 | Phosphoglucomutase | 2.8 | gi|21362784 |
| L27 | Vitamin D-binding protein precursor | 2.0 | gi|46397761 |
| Redox stress/Inflammation | |||
| D1 | Annexin A2 | 8.2 | gi|13435564 |
| D6 | Interleukin 1 receptor accessory | 4.2 | gi|19882203 |
| D9 | Peroxiredoxin 6 | 3.7 | gi|6671549 |
| D10 | GlutathioneS-transferase, pi1 | 3.5 | gi|10092608 |
| D15 | MAP kinase kinase 3 | 2.9 | gi|1771303 |
| D21 | A-kinase anchor protein | 2.3 | gi|2852699 |
| D22 | Glutathione S-transferase Mu 5 | 2.3 | gi|1346207 |
| D23 | Electron transfer flavoprotein alpha-subunit | 2.2 | gi|21759113 |
| D24 | DC-SIGN related protein 1 | 2.2 | gi|46395849 |
| D30 | Selenium bind protein 2 | 0.5 | gi|9507079 |
| E1 | Selenium bind protein 2 | 6.9 | gi|9507079 |
| E6 | Peroxiredoxin 6 | 3.6 | gi|3219774 |
| E7 | G-protein coupled receptor | 3.1 | gi|460318 |
| E12 | Interleukin 6 receptor, alpha | 2.5 | gi|7110655 |
| E13 | Glutathione S-transferase theta 2 | 2.4 | gi|81916034 |
| E16 | Glucocorticoid-attenuated response gene 49 protein | 2.2 | gi|6831574 |
| E18 | Peroxiredoxin 4 | 2.1 | gi|3024715 |
| A5 | Superoxide dismutase 1, soluble | 4.7 | gi|45597447 |
| A11 | Cyclophilin A | 2.3 | gi|118105 |
| A20 | Selenium bind protein 2 | 0.5 | gi|9507079 |
| L4 | Glutathione peroxidase 1 | 4.7 | gi|84871986 |
| L6 | Interferon regulatory factor | 3.6 | gi|972949 |
| L7 | Adrenoleukodystrophy protein | 3.6 | gi|6651050 |
| L8 | Glutathione S-transferase Mu 1 | 3.4 | gi|121716 |
| L15 | Glutathione transferase omega-1 | 2.7 | gi|6016174 |
| L19 | Heat shock protein 60 | 2.4 | gi|51702252 |
| L20 | Mitogen-activated protein kinase 10 | 2.3 | gi|2499604 |
| L23 | Farnesoid X-activated receptor | 2.2 | gi|21263825 |
| L25 | Peroxiredoxin 4 | 2.1 | gi|3024715 |
| Enzyme | |||
| D2 | Glycine N-methyltransferase | 7.1 | gi|6754026 |
| D5 | DEAD-box RNA helicase | 4.3 | gi|6014946 |
| D7 | Cyp2c70 protein | 3.8 | gi|19387996 |
| D8 | 3’-phosphoadenosine-5’-phosphosulfate synthase 1 | 3.7 | gi|6754982 |
| D16 | Isopentenyl-diphosphate delta-isomerase 1 | 2.7 | gi|13878548 |
| D19 | Indolethylamine N-methyltransferase | 2.4 | gi|731019 |
| D27 | Homogentisate 1,2-oxygenase | 2.1 | gi|7387755 |
| D28 | Thiopurine S-methyltransferase | 2.0 | gi|6094505 |
| E2 | Dehydrodolichyl diphosphate synthase | 5.9 | gi|46395956 |
| E20 | Thiopurine S-methyltransferase | 2.1 | gi|6094505 |
| A17 | Ubiquitin carboxyl-terminal hydrolase L1 | 0.4 | gi|20178168 |
| A19 | Hypoxanthine guanine phosphoribosyl transferase 1 | 0.5 | gi|13435621 |
| L2 | Amine N-sulfotransferase | 5.1 | gi|81870419 |
| L11 | 3-hydroxyanthranilate 3,4-dioxygenase | 2.9 | gi|3929397 |
| L12 | Caramoyl-phosphate synthase I | 2.8 | gi|117492 |
| L17 | Era-like-GTPase | 2.5 | gi|19852066 |
| L18 | Betaine-homocysteine S-methyltransferase | 2.5 | gi|5915784 |
| L26 | Thiopurine S-methyltransferase | 2.0 | gi|6094505 |
| Miscellaneous | |||
| D3 | Hypothetical protein LOC74919 | 6.8 | gi|33563309 |
| D4 | Chaperonin groEL precursor | 5.4 | gi|72957 |
| D11 | EF hand domain | 3.4 | gi|13386360 |
| D12 | Growth-arrest-specific protein 2 | 3.4 | gi|120945 |
| D18 | Glial fibrillary acidic protein | 2.6 | gi|417050 |
| D29 | Protein disulfide-isomerase A6 precursor | 0.4 | gi|2501206 |
| E3 | RB-associated-KRAB repressor | 5.1 | gi|29789126 |
| E4 | Hypothetical protein DKFZp547P193.1 | 4.3 | gi|11346331 |
| E5 | Myc-binding protein-associated protein | 3.7 | gi|24762234 |
| E11 | SEC63 | 2.6 | gi|31981948 |
| E14 | START domain | 2.4 | gi|9910482 |
| E15 | Growth-arrest-specific protein 2 | 2.2 | gi|120945 |
| E17 | Alpha-soluble NSF attachment | 2.2 | gi|17380315 |
| E19 | Beta-actin | 2.1 | gi|46397316 |
| E21 | Selenium bind protein 1 | 2.1 | gi|22164798 |
| A1 | Procollagen, type XXIII, alpha 1 | 9.8 | gi|23510253 |
| A2 | Advillin | 8.3 | gi|6857753 |
| A3 | Putative TDPOZ1 protein | 7.7 | gi|38077962 |
| A4 | Serum albumin precursor | 7.2 | gi|5915682 |
| A6 | Hypothetical protein LOC225913 | 4.5 | gi|21703976 |
| A7 | Growth-arrest-specific protein 2 | 3.4 | gi|120945 |
| A8 | Beta-actin | 3.1 | gi|46397316 |
| A9 | START domain | 2.8 | gi|9910482 |
| A15 | Chimerin 1 isoform 2 | 0.4 | gi|13386436 |
| A16 | Rab GDP dissociation inhibitor beta | 0.4 | gi|1707891 |
| A18 | Rho GDP dissociation inhibitor 1 | 0.4 | gi|21759130 |
| L1 | Upp2 protein | 5.3 | gi|20071298 |
| L5 | Eukaryotic translation initiation factor 3 subunit 2 | 3.9 | gi|20138778 |
| L13 | Growth-arrest-specific protein 2 | 2.8 | gi|120945 |
| L16 | Regucalcin | 2.6 | gi|2498920 |
| L21 | Caspase-6 precursor | 2.3 | gi|2493529 |
| L22 | Guanosine diphosphate dissociation inhibitor 2 | 2.2 | gi|13638229 |
| L24 | Transducin alpha-1 chain | 2.1 | gi|121033 |
| L28 | Apolipoprotein A-IV precursor | 2.0 | gi|1703331 |
(a) The spot numbers correspond to the same protein signal that was detected in the Figure 1.
(b) The protein names and accession numbers were taken from the NCBI database.
(c) Ratio of the protein intensity in each PUFA-treated group to the corresponding spot in the control group represents average values of triplicate samples.