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. 2015 Feb 17;10(2):e0117914. doi: 10.1371/journal.pone.0117914

Fig 6. ERK5 phosphorylation at Thr732 via ERK1/2 is required for MEF2C-dependent transcription in HEK293 cells.

Fig 6

(A) HEK293 cells were co-transfected with pMEF2/Luc and β-galactosidase driven by the β-actin promoter. The cells were incubated with EGF (100 ng/ml) in the presence or absence of U0126 (30 μM) for 6 h, and luciferase assays were performed. Values represent the means ± SEM. *P< 0.05 compared with (-) and # P < 0.05 compared with (-) or EGF alone (n = 6). (B) HEK293 cells were co-transfected with pMEF2/Luc, β-galactosidase driven by β-actin promoter, and mycERK5 wild-type (WT) or mycER–32A. The cells were incubated with EGF (100 ng/ml) for 6 h, and luciferase assays were performed. Values represent the means ± SEM. *P < 0.05 compared with (-) and # P < 0.05 compared with ERK5-WT (n = 6).