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. 2015 Feb 17;10(2):e0117444. doi: 10.1371/journal.pone.0117444

Fig 5. riok-1(RNAi) sterility is associated with an oogenesis defect.

Fig 5

(A) Total average brood size for wild-type worms knocked down for riok-1, riok-2 or riok-3 at 20°C, 23°C and 25°C. riok-1(RNAi) worms have a significant reduction in brood size at 20°C and are sterile at 23°C and 25°C. Error bars represent the SEM; n ≥6. **** = P values <0.0001. *** = P values 0.0003 for riok-3 at 25°C (B) Average number of progeny produced in 17 hours from crossing fog-2(q71) male and female worms. Crosses were; control, ♀ fog-2(q71) x ♂ fog-2(q71); ♀ fog-2(q71) x ♂riok-1(RNAi); ♀ fog-2(q71)riok-1(RNAi) x ♂ fog-2(q71). Thirteen individual crosses per treatment were conducted at 25°C with 10 males mated per L4 female. **** = P <0.0001. (C) Knockdown of riok-1 in rrf-1 worms resulted in significantly reduced progeny. Total brood size for rrf-1 worms grown on riok-1 and pCB19 (negative control) RNAi at 25°C. Error bars represent SEM. n = 23, **** = P <0.0001.