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Tissues from PD and healthy volunteers were snap-frozen and incubated with polyclonal antibody against (A) K6F or (B) ScK6F (5 μg/ml). C: Negative immunostaining for K6F in healthy and PD patients by isotype-matched control IgG. Arrow and arrowhead in Panel A(c) indicate K6F immunoreactivity on endothelial cells and the basal cell layer, respectively. Data are representative of three independent experiments. Scale bar, 50 micron.
