Fig 5. In vitro IL-4 and IFN-γ production by splenocytes of vaccinated mice stimulated by HBsAg or PD.

(A) IL-4 responses determined by ELISPOT assay. 1–2, HBsAg and PD-specific IL-4 responses of splenocytes from the conjugated vaccine group, respectively; 3–4, HBsAg-specific IL-4 responses of splenocytes isolated respectively from the commercial vaccine and HBsAg alone groups; 5, PBS group. (B) IFN-γ responses determined by ELISPOT assay. 1–2, HBsAg and PD-specific IFN-γ responses of splenocytes from the conjugated vaccine group, respectively; 3–4, HBsAg-specific IFN-γ responses of splenocytes isolated respectively from the commercial vaccine and HBsAg alone groups; 5, PBS group. The symbol “*” represents statistical significant difference between the groups in the segment ends.