Table 1. Bacterial 16S rRNA pyrosequencing parameters, statistical estimators and gene abundance in different depths of the mofette and reference soils in June 2011.
| Sample | No. of sequencesa | No. of OTUsb | Coverage (%) | Inverse Simpson's (1/D)c | Chao 1 | Shannon-Wienerc | Bacterial 16S rRNA gene copies (g (dw)−1)d |
|---|---|---|---|---|---|---|---|
| Mofette soil | |||||||
| 0–10 cm | 1098 (3747) | 82 | 97 | 2.7 (2.5; 3.0) | 126 (105; 173) | 2.1 (2.0; 2.2) | 7.4±0.9 × 109 |
| 10–25 cm | 1098 (2200) | 124 | 95 | 5.2 (4.7; 6.0) | 154 (140; 182) | 3.0 (2.8; 3.1) | 4.9±0.1 × 109 |
| 25–40 cm | 1098 (4392) | 73 | 97 | 2.5 (2.3; 2.7) | 149 (113; 226) | 2.0 (1.8; 2.1) | 3.8±0.6 × 109 |
| Reference soil | |||||||
| 0–10 cm | 1098 (1098) | 177 | 95 | 19.8 (17.4; 23.0) | 200 (188; 223) | 4.0 (3.9; 4.1) | 1.6±0.2 × 1010 |
| 10–25 cm | 1098 (1577) | 184 | 93 | 16.2 (14.3; 18.8) | 229 (206; 269) | 3.9 (3.8; 4.0) | 4.9±0.4 × 109 |
| 25–40 cm | 1098 (2481) | 213 | 92 | 25.2 (22.0; 29.6) | 288 (258; 340) | 4.3 (4.2; 4.4) | 5.4±0.3 × 108 |
a
Number of denoised sequences before normalization is given in brackets.
b
97% sequence similarity cutoff.
c
Higher numbers indicate more diversity; numbers in parentheses are 95% confidence intervals.
d
Mean±s.d.