Table 2. Archaeal 16S rRNA pyrosequencing parameters, statistical estimators and gene abundance in different depths of the mofette and reference soils in June 2011.
| Sample | No. of sequencesa | No. of OTUsb | Coverage (%) | Inverse Simpson's (1/D)c | Chao 1 | Shannon-Wienerc | Archaeal 16S rRNA gene copies (g (dw)−1)d |
|---|---|---|---|---|---|---|---|
| Mofette soil | |||||||
| 0–10 cm | 545 (545) | 38 | 98 | 4.3 (3.9; 4.8) | 45 (40; 62) | 2.0 (1.9; 2.1) | 7.7±0.5 × 108 |
| 10–25 cm | 545 (3624) | 22 | 98 | 1.9 (1.8; 2.1) | 29 (24; 54) | 1.2 (1.1; 1.3) | 5.2±0.5 × 108 |
| 25–40 cm | 545 (4353) | 23 | 98 | 2.7 (2.4; 3.0) | 32 (25; 60) | 1.4 (1.3; 1.5) | 4.3±0.3 × 108 |
| Reference soil | |||||||
| 0–10 cm | 545 (2089) | 66 | 93 | 4.2 (3.7; 4.9) | 121 (91; 187) | 2.2 (2.1; 2.4) | 3.1±0.2 × 108 |
| 10–25 cm | 545 (676) | 70 | 94 | 8.4 (7.5; 9.6) | 93 (80; 126) | 2.8 (2.7; 2.9) | 2.7±0.1 × 108 |
| 25–40 cm | 545 (1434) | 67 | 94 | 10.4 (9.3; 11.9) | 98 (80; 141) | 2.9 (2.8; 3.0) | 7.6±0.4 × 106 |
a
Number of denoised sequences before normalization is given in brackets.
b
97% sequence similarity cutoff.
c
Higher numbers indicate more diversity; numbers in parentheses are 95% confidence intervals.
d
Mean±s.d.