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. 2015 Feb 18;35(7):3001–3009. doi: 10.1523/JNEUROSCI.3678-14.2015

Figure 1.

Figure 1.

Progressive cerebellar granule neuron degeneration in nm2453 mutant mice. A–H, Sagittal sections of cerebella from 2-month-old (A, C), 11-month-old (B, D), and 17-month-old (E, G) nm2453−/− and 26-month-old wild-type (WT; F, H) mice were stained with hematoxylin and eosin. Higher-magnification images of lobule II are shown (C, D, G, H). Note the presence of vacuoles in the 11- and 17-month-old mutant cerebella. I,The number of granule cells in cerebellar lobule II from the indicated ages. Results are shown as mean ± SEM (n = 3). *p < 0.001 (statistically significant differences between similarly aged mutant and WT mice and aged and 2-month-old mutant mice). J, K, Immunohistochemistry with antibodies to calbindin D-28 on cerebellar sections from 17-month-old nm2453−/− (J) and 18-month-old WT mice. Note the intact Purkinje cell layer in the mutant cerebellum. Cerebellar lobules are indicated by Roman numerals (F). Scale bars: F, 500 μm; H, 50 μm; K, 1 mm.