Figure 4.

H4K16ac, a downstream target of SIRT1, is regulated in NAc by cocaine. A, ChIP-seq reveals prominent binding of H4K16ac to promoter/gene body regions (∼80%) in NAc, with much less binding to nongenic regions. Pie charts represent genome-wide occupancy of H4K16ac in saline (16,506 peaks) and chronic cocaine (15,562 peaks) conditions. B, H4K16ac binding to gene regions genome-wide, as visualized by ngs.plot, in NAc under saline and chronic cocaine conditions. C, Analysis of cocaine-induced changes in H4K16ac binding genome-wide, showing most regulation in promoter and gene body regions. D, Venn diagram showing genes that display both a decrease in SIRT1 binding and an increase in H4K16ac binding in NAc after chronic cocaine.