Table 1.
Plasmids used in this study
| Plasmid name | Cloning technique | Cloning sites | Feature | Tags | Source |
|---|---|---|---|---|---|
| pIRES2-EGFP | Starting vector | Clontech | |||
| pIRES2-EGFP-CHMP2B WT | In-Fusion (Clontech) | XhoI, XhoI | RNAi res. | C-terminal FLAG | This study |
| pBI-CMV3 | Starting vector | Clontech | |||
| pBI-CMV3-CHMP2B WT | In-Fusion (Clontech) | BamHI, HindIII | RNAi res. | This study | |
| pBI-CMV3-CHMP2B R19/22/26A | In-Fusion (Clontech) | BamHI, HindIII | RNAi res. | This study | |
| pBI-CMV3-CHMP2B L4D/F5D | In-Fusion (Clontech) | BamHI, HindIII | RNAi res. | This study | |
| pBI-CMV3-CHMP2B L207D/L210D | In-Fusion (Clontech) | BamHI, HindIII | RNAi res. | This study | |
| pSuper-mCherry | Starting vector | Belly et al., 2010 | |||
| pSuper-mCherry shRNA vs CHMP2B | Standard ligation | HindIII, BglII | Belly et al., 2010 | ||
| pSuper-mCherry shRNA vs GFP | Standard ligation | HindIII, BglII | This study | ||
| pCDNA3.1-CHMP2B WT | Topo (Invitrogen) | C-terminal HA | This study | ||
| pCDNA3.1-CHMP2B R19/22/26A | Topo (Invitrogen) | C-terminal HA | This study | ||
| pCDNA3.1-CHMP4B | Topo (Invitrogen) | C-terminal FLAG | Bodon et al., 2011 |
The cloning technique used to construct each plasmid and, if applicable, the associated kit supplier are indicated. RNAi res. Indicates the encoded mRNA is resistant to RNAi by the Chmp2b shRNA.