Table 1.
Antisera used to characterize IP3R3 MV cells
| Antisera against | Marker for | Company | Lot | |
|---|---|---|---|---|
| Acetylated tubulin | olfactory cilia | Sigma T6793 | 041K4817 | − |
| AC III | component of olfactory transduction pathway | Santa Cruz sc-588 | J3009 | − |
| CGRP | trigeminal nerve fibers (contacting IP3R3 cells) | Peninsula Lab. T-4032 | 040826-4 | +/− |
| ChAT | enzyme that synthesises acetylcholine | Abcam AB65097 Chemicon AB144P |
634985 LV1443701 LV1580976 |
− |
| CK18 | supporting cells | Chemicon MAB3234 Bioworld Techn.,Inc. BS1204 |
0507004430 380704 |
− − |
| CNG2A | component of olfactory transduction pathway | Santa Cruz sc-13700 | – | − |
| Espin | actin-binding protein in microvilli | Dr. J. Bartles, Northwestern University, Chicago | – | − |
| Gαolf | component of olfactory transduction pathway | Santa Cruz sc-383 | L0707 | − |
| Gαq/11 | G-protein subunit q/11/14 – transduction component | Santa Cruz Sc-392 | F1107 | + |
| GFP | green fluorescent protein | Abcam AB290 | 207431 | + |
| Gustducin | G-protein subunit in taste and solitary chemosensory cells | Santa Cruz sc-395 | D0808, D181, E1704 | − |
| Hu-C | neuronal cells | Molecular Probes A21271 | A21271 | − |
| IP3R3 | IP3 receptor 3 – transduction component | Chemicon AB9076 | 25041643 | + |
| OMP | olfactory sensory neurons | Dr. F. Margolis, University of Maryland | – | − |
| P2X2 | purinergic receptor – transduction component | Alomone Labs APR003 | AN-06 | − |
| P2X3 | purinergic receptor – transduction component | Chemicon AB5895 | 0602021455 | +/− |
| PDE2A | transduction component | Santa Cruz Sc-17227 | L0402 | − |
| PGP 9.5 | neuronal and neuroendocrine cells | AbD Serotec 7663-0504 | 071207 | − |
| PLCβ2 | transduction component | Santa Cruz Sc-206 | I181, A1204, B0907 | − |
| Substance P | trigeminal nerve fibers (contacting IP3R3 MV cells) | Accurate YMC1021 | E9381 | +/− |
| SUS-1 | supporting cells in the MOE | Dr. James Morgan Roche Res. Ctr., Nutley, N.J. | 17S-4-III-83 | − |
| TH | Tyrosine hydroxylase | PhosphoSolutions 2025THRAB | ajo209 | − |
| TrpC6 | transient receptor potential channel C6 – transduction component | Abcam AB12249 | 342108 | +/− |
| VAChT | membrane transport protein | Chemicon AB1578 | 24080681 | − |
| Villin | actin-binding protein in microvilli | Beckman-Coulter (ImmunoTech) 0258 | 1D2C3 | − |
+
= expressed in all IP3R3 MV cells
+/−
= expressed in some IP3R3 MV cells or nerve fibers
−
= not expressed in IP3R3 MV cells)