Fig. 2.

(A) Representative negative stain AcrB classes. The classes are predominantly of a “side” view, equivalent to that viewed from the plane of the bilayer, with an example of a “base” view from the cytoplasmic surface of the protein shown in the far right panel (vii). B) Representative AcrB singlet classum on the left, with some of the raw particles which make up the class shown. C) Classes of the AcrB trimer doublets (top panel), with the orientations shown below (bottom panel) using the AcrB reconstruction. Scale bar represents 15 nm.