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. 2015 Jan;1850(1):13–21. doi: 10.1016/j.bbagen.2014.09.023

Fig. 1.

Fig. 1

Expression of Mtb H37Rv ADP-Glc PPase with the vector pMIP12 in M. smegmatis mc2155. (A) Activity histogram of soluble samples. (B) Immunodetection of ADP-Glc PPase of corresponding samples in A after SDS-PAGE and immunobloting. The lanes are defined as follows: pMIP12, crude extracts from M. smegmatis mc2155 cells transformed with pMIP12 (empty vector control); pMIP12/glgC, crude extracts from M. smegmatis mc2155 cells transformed with pMIP12/MtbglgC; GlgC, purified ADP-Glc PPase; WB control, denatured/solubilized pellet from E. coli BL21cells transformed with pET19/MtglgC. Samples were assayed for activity in the direction of ADP-Glc synthesis, as stated under Materials and methods for Assay B.