Fig. 5.

Sterol composition of the pdr16Δ strain overexpressing wild-type Pdr16p and PI binding deficient Pdr16p ^{E235A, K267A}. pdr16Δ strain containing either the empty multicopy cloning vector YEplac181, the wild-type PDR16 allele in YEplac181 plasmid or mutant pdr16^{E235A, K267A} allele in YEplac181 plasmid together with the parental wild-type strain FY1679-28c were pre-grown in synthetic yeast media without leucine as required for plasmid selection. Subsequently, they were grown for 6 hours at 28 °C in YEPD media without miconazole (A) or with sub-inhibitory concentration of miconazole (5 ng/ml) (B). Following extraction non-saponifiable lipids were analyzed by HPLC as described in Materials and methods. Total sterols represent ergosterol and its precursors including lanosterol, and squalene. Data represent mean ± S.E.M. from three experiments.