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. 2015 Feb;1851(2):117–128. doi: 10.1016/j.bbalip.2014.11.005

Fig. 4.

Fig. 4

Ethanolamine phospholipids of RCDP fibroblasts have normal levels of total PUFAs but are enriched in arachidonic acid. (A) The composition of the sn-2 side chains was determined in PlsEtn of the different fibroblast groups and the concentrations of the individual species were calculated as percentage of total PlsEtn. To enable better comparison, relative values for the most abundant species are shown with those of the control group normalized to 100%. Values not normalized to controls for all measured species are shown in Supp. Fig. 1. *P ≤ 0.05 (one-way ANOVA with Tukey's post hoc test; Bonferroni–Holm correction for multiple testing) (B) PUFA side chains were added up in PlsEtn and PE. Horizontal lines in the bars representing total PUFA species indicate the different contributions of PE and PlsEtn. Individual species contributing to the PUFA group are listed in Section 2.7. ***P ≤ 0.001; *P ≤ 0.05; n.s., not significant (one-way ANOVA with Tukey's post hoc test). P ≥ 0.98 for all comparisons of total PUFA levels (C) PE species were grouped according to the number of double bonds in their side chains into PE with 4–5 double bonds (mostly AA-containing) or PE with 6–7 double bonds (mostly DHA-containing). Data represent means ± SD of at least three different cell lines. ***P ≤ 0.001; *P ≤ 0.05; n.s., not significant (one-way ANOVA with Tukey's post hoc test).