Figure 1.

Body weight, food intake, glucose tolerance, lean mass, fat mass, and physical activity levels of mice on methionine restriction (MR) and control diet. (A) Body weight measurements from the first 5 weeks of the study, (B) food intake from a representative 4-week period of the study, and (C) glucose tolerance as assessed by a glucose tolerance test (dose of glucose = 2 g kg⁻¹) after a 5-h fast in mice fed MR or control diet at the ages of 2 months (n = 20) and 12 months (n = 14). Significance was calculated by repeated measures two-way ANOVA with Bonferroni multiple comparison post hoc tests. (D) Lean mass (g), (E) fat mass (g), and (F) % fat mass of total body mass were measured by dual-energy X-ray absorptiometry in mice fed MR or control diet at the ages of 2 months (n = 20) and 12 months (n = 14). Significance was calculated by one-way ANOVA and Tukey’s post hoc test. (G) Physical activity was measured by beam breaks per minute in mice fed MR or control diet at the ages of 2 months (n = 6) and 12 months (n = 6). Significance was calculated by two-tailed Student’s t-test. Data are represented as mean ± SEM. Black bars/circles, 12-month-old control-fed mice; white bars/circles, 12-month-old MR-fed mice; black hatch bars/black triangles, 2-month-old control-fed mice; white hatch bars/white triangles, 2-month-old MR-fed mice. *MR-fed mice significantly different to age-matched control-fed mice (P < 0.05), #12-month-old control-fed mice significantly different to 2-month-old control-fed mice (P < 0.05).