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. 2014 Jul 18;13(5):850–861. doi: 10.1111/acel.12245

Figure 7.

Figure 7

The effects of estrogen stimulation on miRNA transcripts and phosphorylation of AKT protein in young male mouse diaphragm muscle. (A) qPCR analyses of miRNA transcripts normalized to U6B in mouse muscle stripes treated with 100 nm estradiol or mock for 24 h. (B) Western blot analysis of phosphorylated AKT and total AKT in mouse muscles treated with 100 nm estradiol for 24 h. The amount of phosphorylated AKT was analyzed by densitometry and normalized against AKT expression. Data are presented as percentage of control (mock) and reported as means ± SD of three independent experiments. OD indicates optical density. t-test, *P < 0.05.