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. 2013 Dec 6;13(2):329–339. doi: 10.1111/acel.12179

Figure 5.

Figure 5

EGR-1 activates egl-27 expression, but neither egl-27 nor egr-1 regulate egr-1 expression. (A) Worms expressing egl-27pro:mCherry were placed on egr-1 RNAi, egl-27 RNAi, or empty vector control RNAi as eggs and imaged 3 days later as day 1 adults. egl-27 RNAi decreased egl-27 promoter activity by 22% (**p < 0.01), and egr-1 RNAi decreased egl-27 promoter activity by 40% (***p < 0.001). All data are represented as mean fluorescence intensity in arbitrary units; error bars are ±SEM. (B) Worms expressing EGR-1:GFP in the glo-4(ok623) background were placed on either egl-27 RNAi or empty vector control RNAi as eggs and imaged 3 days later as day 1 adults. There was no significant difference in EGR-1 expression in either group. (C) Worms expressing egr-1pro:mCherry were placed on either egr-1 RNAi or empty vector control RNAi as eggs and imaged 3 days later as day 1 adults. There was no significant difference in egr-1 expression in either group.