Table 3.
Statistics of the assembling process
| Step 1 | Step 2 | Step 3 | Step 4 | Step 5 | Step 6 | Step 7 | |
|---|---|---|---|---|---|---|---|
| # Minigaps | 27 | 27 | 15 | 13 | 1 | 0 | 0 |
| Assembled nt in the big gap | 0 | 0 | 12,511 | 12,511 | 12,511 | 35,746 | 35,746 |
| Total nt assembled* | 1,808,353 | 1,808,353 | 1,828,147 | 1,828,363 | 1,832,588 | 1,851,716 | 1,851,618 |
*: "N"s are not counted. Assemblies in Steps 1-6 have overlapping end sequences (terminal redundancy). As a result, the assembly in Step 6 appeared to be slightly bigger than the final assembly.