Fig. 1. Galectin-3 expression correlated with 5-FU susceptibility in human colon cancer cell lines and gene expression profiling linked to galectin-3.

(A) Galectin-3 protein expression correlated with 5-FU susceptibility in three human colon cancer cell lines, SNU-769B, SNU-C4 and SNU-C5. Whole proteomes obtained from the human colon cancer cell lines employed were subjected to SDS-PAGE and were electro-transferred to PVDF membranes for western blot analysis. When galectin-3 expression was higher, human colon cancer cell lines showed more 5-FU susceptibility. (B) Gene expression profiling liked to galectin-3. To satisfy minimum clustering sample size, we added SNU-61, which has almost the same 5-FU susceptibility as SNU-769B, and as shown in the enlarged yellow box, genes linked to galectin-3 expression were selected (a). The expressional profiling was further confirmed by using real-time PCR as shown in panel (b). All genes showing positive and negative expressional correlations with galectin-3 are listed in Tables2, 3respectively.