Fig. 3. Reduced GABABR1 expression and suppressed cell proliferation of SNU-C4 by treatment with HAD-B.

(A) Decreased GABABR1 expression by treatment with HAD-B. At 96 hrs after treatment of with 1 mg/ml HAD-B, protein expression of GABABR1 was decreased in SNU-C4. (B) Suppressed cell proliferation by treatment with HAD-B. GABA treatment recovered the rate of proliferation of SNU-C4 that had been suppressed by HAD-B treatment. (C) Increase in the intracellular cAMP by either GABA or HAD-B treatment. Treatment with GABA or HAD-B increased the basal level of intracellular cAMP.