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| Mobile phase | ||||
|---|---|---|---|---|
| Time (min) |
Flow (ml/min) |
A (Buffer/ACN) 95 / 5 Buffer: 10 mM KH2 PO4 (Adjusted to pH 2.5 with phosphoric acid) |
B (ACN) | |
| Gradient profile | 0 | 0.8 | 100 | 0 |
| 30 | 0.8 | 70 | 30 | |
| 40 | 0.8 | 70 | 30 | |
| 60 | 0.8 | 10 | 90 | |
| 70 | 0.8 | 100 | 0 | |
| 90 | 0.8 | 100 | 0 | |
