Table. 2. Content analyses of the Bufonis Venomum by using HPLC for different extraction methods.
| Extraction method | Sample | Constituent | Area | Content (㎎/g) |
|---|---|---|---|---|
| Evaporation after 80%
MeOH extraction |
80% MeOH ext. | Serotonin | 776.3 ± 12.0 | 35.4 ± 1.2 |
| Cinobufotalin | 202.0 ± 8.4 | 8.4 ± 0.5 | ||
| Bufalin | 228.2 ± 10.2 | 11.7 ± 0.4 | ||
| Resinobufogenin | 253.6 ± 9.9 | 20.9 ± 0.4 | ||
| Cinobufagin | 463.9 ± 32.7 | 27 ± 2.1 | ||
| EtOAc Ext | Serotonin | 19.4 ± 1.2 | 0.3 ± 0.0 | |
| Cinobufotalin | 879.7 ± 61.6 | 16.9 ± 0.9 | ||
| Bufalin | 1059.7 ± 67.3 | 26.3 ± 0.4 | ||
| Resinobufogenin | 1268.5 ± 50.2 | 35.8 ± 0.3 | ||
| Cinobufagin | 2145.2 ± 22.7 | 43.8 ± 0.1 | ||
| Freeze dry after water extraction | Water Ext | Serotonin | 1228.1± 20.9 | 75.7 ± 0.1 |
| Cinobufotalin | 112.6 ± 2.7 | 1.5 ± 0.0 | ||
| Bufalin | 57.8 ± 0.6 | - | ||
| Resinobufogenin | 46.2 ± 2.1 | 0.8 ± 0.0 | ||
| Cinobufagin | 105.9 ± 8.4 | 1.5 ± 0.0 | ||
| EtOAc Ext | Serotonin | - | - | |
| Cinobufotalin | 632.1 ± 21.8 | 2.8 ± 0. | ||
| Bufalin | 363.7 ± 7.1 | 1.8 ± 0.0 | ||
| Resinobufogenin | 293.1 ± 4.3 | 1.8 ± 0.0 | ||
| Cinobufagin | 624.3 ± 8.9 | 2.8 ± 0.0 |