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. 2012 Dec;15(4):52–65. doi: 10.3831/KPI.2012.15.012

Table. 3. Content analyses of the Bufonis Venomum by using HPLC after extraction dehydration.

Constituent Area Content (㎎/g)
EtOH Ext Serotonin 303.4 ± 11.0 1.0 ± 0.0
Cinobufotalin 9319.4 ± 21.3 43.9 ± 1.7
Bufalin 13256.9 ± 33.6 80.8 ± 1.3
Resinobufogenin 24321.8 ± 44.2 158.5 ± 6.5
Cinobufagin 16075.3 ± 55.8 76.0 ± 0.3
EtOH Ext (hot-water reflux) Serotonin - -
Cinobufotalin 6565.6 ± 20.9 25.3 ± 0.2
Bufalin 9706.6 ± 12.7 48.5 ± 0.3
Resinobufogenin 20068.7 ± 33.7 107.3 ± 1.1
Cinobufagin 11818.6 ± 93.5 45.8 ± 0.7
EtOH Ext (precipitation) Serotonin - -
Cinobufotalin 6200.3 ± 66.1 5.6 ± 0.1
Bufalin 9081.9 ± 21.6 10.6 ± 0.1
Resinobufogenin 19757.9 ± 93.5 24.7 ± 2.7
Cinobufagin 11545.4 ± 95.8 10.5 ± 0.2
EtOH Ext (hot-water reflux) Serotonin 303.0 ± 10.2 0.8 ± 0.0
Cinobufotalin 7939.3 ± 82.4 31.8 ± 0.1.
Bufalin 11826.3 ± 33.6 61.4 ± 0.1
Resinobufogenin 23067.6 ± 212.1 128.0 ± 0.2
Cinobufagin 14320.4 ± 77.3 57.6 ± 0.3
EtOH Ext (precipitation) Serotonin 45.8 ± 1.0 0.1 ± 0.0
Cinobufotalin 9086.5 ± 43.1 31.5 ± 0.1
Bufalin 13695.0 ± 77.9 61.6 ± 0.2
Resinobufogenin 25570.1 ± 101.5 123.0 ± 0.2
Cinobufagin 16807.7 ± 21.9 58.7 ± 0.1