Table. 3. Content analyses of the Bufonis Venomum by using HPLC after extraction dehydration.
Constituent | Area | Content (㎎/g) | |
---|---|---|---|
EtOH Ext | Serotonin | 303.4 ± 11.0 | 1.0 ± 0.0 |
Cinobufotalin | 9319.4 ± 21.3 | 43.9 ± 1.7 | |
Bufalin | 13256.9 ± 33.6 | 80.8 ± 1.3 | |
Resinobufogenin | 24321.8 ± 44.2 | 158.5 ± 6.5 | |
Cinobufagin | 16075.3 ± 55.8 | 76.0 ± 0.3 | |
EtOH Ext (hot-water reflux) | Serotonin | - | - |
Cinobufotalin | 6565.6 ± 20.9 | 25.3 ± 0.2 | |
Bufalin | 9706.6 ± 12.7 | 48.5 ± 0.3 | |
Resinobufogenin | 20068.7 ± 33.7 | 107.3 ± 1.1 | |
Cinobufagin | 11818.6 ± 93.5 | 45.8 ± 0.7 | |
EtOH Ext (precipitation) | Serotonin | - | - |
Cinobufotalin | 6200.3 ± 66.1 | 5.6 ± 0.1 | |
Bufalin | 9081.9 ± 21.6 | 10.6 ± 0.1 | |
Resinobufogenin | 19757.9 ± 93.5 | 24.7 ± 2.7 | |
Cinobufagin | 11545.4 ± 95.8 | 10.5 ± 0.2 | |
EtOH Ext (hot-water reflux) | Serotonin | 303.0 ± 10.2 | 0.8 ± 0.0 |
Cinobufotalin | 7939.3 ± 82.4 | 31.8 ± 0.1. | |
Bufalin | 11826.3 ± 33.6 | 61.4 ± 0.1 | |
Resinobufogenin | 23067.6 ± 212.1 | 128.0 ± 0.2 | |
Cinobufagin | 14320.4 ± 77.3 | 57.6 ± 0.3 | |
EtOH Ext (precipitation) | Serotonin | 45.8 ± 1.0 | 0.1 ± 0.0 |
Cinobufotalin | 9086.5 ± 43.1 | 31.5 ± 0.1 | |
Bufalin | 13695.0 ± 77.9 | 61.6 ± 0.2 | |
Resinobufogenin | 25570.1 ± 101.5 | 123.0 ± 0.2 | |
Cinobufagin | 16807.7 ± 21.9 | 58.7 ± 0.1 |