Table. 2. Retention times and responses data for calibration standards of QUE, BDMC, DMC, and CUR.
| Concentration(μg/mL) | Retention time (n = 5) | Peak area (n = 5) | ||
|---|---|---|---|---|
| Mean (min) | RSD (%) | Mean (min) | RSD (%) | |
| QUE | ||||
| 1.25 | 3.970 | 0.117 | 94937 | 0.676 |
| 5 | 3.972 | 0.066 | 367965 | 0.739 |
| 20 | 3.972 | 0.041 | 1438240 | 0.624 |
| 40 | 3.973 | 0.055 | 2781685 | 0.508 |
| 80 | 3.972 | 0.029 | 5582929 | 0.437 |
| 140 | 3.972 | 0.048 | 9735618 | 0.866 |
| 200 | 3.972 | 0.053 | 14073938 | 0.368 |
| BDMC | ||||
| 1.25 | 13.823 | 0.308 | 1859 | 1.611 |
| 5 | 13.840 | 0.095 | 8843 | 1.181 |
| 20 | 13.842 | 0.093 | 37086 | 1.089 |
| 40 | 13.843 | 0.087 | 71560 | 1.044 |
| 80 | 13.846 | 0.117 | 143659 | 1.073 |
| 140 | 13.846 | 0.134 | 249462 | 1.835 |
| 200 | 13.849 | 0.060 | 363457 | 0.850 |
| DMC | ||||
| 1.25 | 15.214 | 0.227 | 14705 | 0.273 |
| 5 | 15.229 | 0.096 | 52692 | 0.540 |
| 20 | 15.230 | 0.074 | 204602 | 0.665 |
| 40 | 15.232 | 0.073 | 398446 | 0.436 |
| 80 | 15.237 | 0.099 | 798153 | 0.867 |
| 140 | 15.236 | 0.120 | 1384220 | 1.416 |
| 200 | 15.242 | 0.039 | 2015583 | 0.158 |
| CUR | ||||
| 1.25 | 16.708 | 0.199 | 46645 | 0.856 |
| 5 | 16.718 | 0.077 | 182515 | 0.901 |
| 20 | 16.719 | 0.061 | 701982 | 0.700 |
| 40 | 16.720 | 0.064 | 1358591 | 0.299 |
| 80 | 16.725 | 0.096 | 2737751 | 0.423 |
| 140 | 16.725 | 0.108 | 4749355 | 0.897 |
| 200 | 16.734 | 0.067 | 6866971 | 0.313 |
RSD, relative standard deviation; QUE, quercetin; BDMC, bisdemethoxycurcumin; DMC, demethoxycurcumin; CUR, curcumin.