Table. 5. System suitability parameters, calculation formula and recommendations.
| Parameter | Formula | Recommendation |
|---|---|---|
| Precision | RSD = S/x̄*100 | RSD ≤ 1% for n ≥ 5 |
| Resolution, R | R = (tR2– tR1)/(1/2)(tw1–tw2) | > 2 |
| Tailing factor, Tf | Tf = Wx/2f | ≤ 2 |
| Theoretical plates, N | N = 16(tR/tw)2 | Column efficiency ≥ 2000 |
| Capacity factor, k | k’ = (tR– t0)/t0 | > 2 |
S, standard deviation; x̄ , mean of the data; tR, retention time of analyte 1; tw, peak width measured to the baseline of the extrapolated straight sides to baseline; Wx, width of the peak determined at either 5% (0.05) or 10% (0.10) from the baseline of the peak height; f, distance between peak maximum and peak front at Wx; t0, elution time of the void volume or non retained components.