Table. 4a. (a). Robustness – change in organic composition.
| System suitability | Compound | Change in the normal organic composition of acetonitrile: 2% acetic acid | |||||
|---|---|---|---|---|---|---|---|
| (A) Normal condition | (B) 38% : 62% v/v | (C) 42% : 58% v/v | |||||
| Mean (n = 6) | RSD (%) | Mean (n = 6) | RSD (%) | Mean (n = 6) | RSD (%) | ||
| Retention time, tR(minutes) | QUE | 3.993 | 0.690 | 4.251 | 0.155 | 3.761 | 0.040 |
| BDMC | 13.951 | 0.342 | 17.645 | 0.374 | 11.280 | 0.084 | |
| DMC | 15.340 | 0.291 | 19.543 | 0.285 | 12.330 | 0.084 | |
| CUR | 16.829 | 0.245 | 21.617 | 0.296 | 13.464 | 0.082 | |
| Peak area | QUE | 6853044 | 0.433 | 6836934 | 0.441 | 6867445 | 0.117 |
| BDMC | 167417 | 0.647 | 161504 | 0.801 | 146484 | 0.578 | |
| DMC | 940836 | 0.404 | 903191 | 0.781 | 965307 | 0.071 | |
| CUR | 3302593 | 0.236 | 3206134 | 0.555 | 3367309 | 0.114 | |
| Resolution, R | QUE | - | - | - | - | - | - |
| BDMC | 32.498 | 0.379 | 36.449 | 0.471 | 29.120 | 0.063 | |
| DMC | 2.908 | 0.208 | 3.272 | 2.369 | 2.736 | 0.181 | |
| CUR | 2.850 | 0.237 | 3.243 | 1.648 | 2.666 | 0.124 | |
| Tailing factor, Tf | QUE | 1.371 | 0.254 | 1.347 | 0.115 | 1.392 | 0.074 |
| BDMC | 1.533 | 0.364 | 1.283 | 2.314 | 1.080 | 0.200 | |
| DMC | 1.160 | 0.484 | 1.083 | 0.151 | 1.431 | 0.082 | |
| CUR | 1.094 | 0.094 | 1.076 | 0.284 | 1.114 | 0.037 | |
| Theoretical plate, N | QUE | 8752.133 | 1.463 | 8857.791 | 0.312 | 8520.171 | 0.238 |
| BDMC | 15931.889 | 1.147 | 16311.011 | 0.058 | 16303.130 | 0.103 | |
| DMC | 14298.287 | 1.761 | 16569.474 | 1.029 | 14210.321 | 0.233 | |
| CUR | 16008.049 | 1.202 | 16543.754 | 0.535 | 15157.508 | 0.340 | |
| Capacity factor, k’ | QUE | 0.680 | 0.344 | 0.777 | 0.906 | 0.601 | 0.327 |
| BDMC | 4.878 | 0.202 | 3.800 | 0.209 | 3.800 | 0.209 | |
| DMC | 5.463 | 0.232 | 7.214 | 1.592 | 4.247 | 0.206 | |
| CUR | 6.097 | 0.253 | 8.038 | 0.481 | 4.729 | 0.209 | |