Table. 4b. (b). Robustness – change in acetic acid concentration.
| System suitability | Compound | Change in the acetic acid concentration (% v/v ) | |||||
|---|---|---|---|---|---|---|---|
| (A) Normal condition | (B) 1.0% (pH 2.73) | (C) 3.0% (pH 2.48) | |||||
| Mean (n = 6) | RSD (%) | Mean (n = 6) | RSD (%) | Mean (n = 6) | RSD (%) | ||
| Retention time, tR(minutes) | QUE | 3.972 | 0.175 | 4.054 | 0.064 | 3.893 | 0.071 |
| BDMC | 13.868 | 0.310 | 14.549 | 0.086 | 13.177 | 0.167 | |
| DMC | 15.255 | 0.265 | 16.017 | 0.085 | 14.542 | 0.153 | |
| CUR | 16.743 | 0.213 | 17.590 | 0.084 | 16.028 | 0.141 | |
| Peak area | QUE | 7039483 | 0.562 | 6966950 | 0.525 | 6952833 | 0.630 |
| BDMC | 180475 | 0.541 | 176885 | 0.575 | 152439 | 0.895 | |
| DMC | 1000716 | 0.736 | 987128 | 0.551 | 956266 | 0.670 | |
| CUR | 3433379 | 0.754 | 3428762 | 0.533 | 3428762 | 0.558 | |
| Resolution, R | QUE | - | - | - | - | - | - |
| BDMC | 32.327 | 0.172 | 33.254 | 0.244 | 31.950 | 0.268 | |
| DMC | 2.900 | 0.370 | 2.974 | 0.303 | 3.033 | 0.527 | |
| CUR | 2.840 | 0.429 | 2.904 | 0.339 | 2.966 | 0.608 | |
| Tailing factor, Tf | QUE | 1.366 | 0.077 | 1.364 | 0.215 | 1.370 | 0.110 |
| BDMC | 1.493 | 1.377 | 1.463 | 0.331 | 1.060 | 0.139 | |
| DMC | 1.160 | 1.075 | 1.137 | 0.103 | 1.325 | 0.823 | |
| CUR | 1.085 | 0.148 | 1.092 | 0.050 | 1.083 | 0.108 | |
| Theoretical plate, N | QUE | 8711.993 | 0.267 | 8877.546 | 0.460 | 8548.948 | 0.269 |
| BDMC | 15740.557 | 0.397 | 16067.808 | 0.689 | 16308.146 | 0.664 | |
| DMC | 14041.181 | 0.701 | 14691.580 | 0.675 | 14241.082 | 1.031 | |
| CUR | 15793.019 | 0.472 | 16098.239 | 0.701 | 15531.342 | 0.811 | |
| Capacity factor, k’ | QUE | 0.656 | 1.783 | 0.680 | 1.484 | 0.610 | 0.803 |
| BDMC | 4.798 | 1.202 | 5.036 | 0.658 | 4.449 | 0.511 | |
| DMC | 5.333 | 0.988 | 5.637 | 0.698 | 5.014 | 0.478 | |
| CUR | 6.016 | 1.416 | 6.295 | 0.628 | 5.629 | 0.443 | |